One versatile instrument to power innovation

  • Learn how the NX One can streamline your workflows, accelerate your research, and open up new opportunities for discovery.
  • Explore our Application Notes & mammalian and non-mammalian library which highlight examples of the Nodexus NX One instrument, consumables, and software.
  • The NX One simplifies the complexities of single-cell sorting and dispensing at a price anyone can afford.

Cell line & antibody development

Clonal expansion has never been simpler. Use the NX One’s flexible sorting modes to easily isolate the high-producing recombinant cell lines.

Application Notes - Cell Line Development CHO-GFP

Expression-Based Cell Line Development

The cell line development process is essential for biomedical and basic research as it broadly contributes to the efficiency, quality, and consistency of experimental outcomes, e.g., newer therapeutic approaches such as gene therapy require the development of monoclonal cell lines to test the efficacy and toxicity of viral vector transduction

CRISPR & gene editing

Optimize your gene editing efficiency by detecting and isolating transfected cell populations using the NX One’s highly-specific 1- to 3-color detection.

Application Notes - Gentle and Efficient Single-Cell Sorting - CHO

Gentle, Efficient, and Reproducible Single Cell Sorting of Human iPSCs

Human induced pluripotent stem cells (iPSCs) hold immense significance and potential in regenerative medicine and disease modeling. These cells, derived from adult somatic cells like the skin or blood cells, can be reprogrammed to revert to a pluripotent state, which enables them to differentiate into any cell type in the human body (Mahajani et al., 2019)

Cell & gene therapy

Protect the sterility and minimize cross-contamination of your precious samples using the NX One’s individually-packed, disposable cartridges.

Application Notes - Optimizing Single Cell Sorting and Input Specifications

Optimizing Single Cell Sorting: Input Concentrations and Ratios

The central objective of single-cell sorting is to isolate specific cell types from larger populations to elucidate their function, which is essential for understanding the role they play in disease development and for deriving potential therapeutic approaches

Single-cell genomics & RNA-Seq

Isolate single cells with minimal perturbation to biology for your downstream analysis and ‘omics needs.

Application Notes - University of Chicago Poster

Nodexus – University of Chicago Poster

Gentle stem cell isolation

Preserve the viability of fragile cell populations that typically suffer through conventional sorting methods by leveraging the NX One’s gentle operating pressures (less than 1 psi) for sorting and dispensing.

Application Notes - Gentle and Efficient hiPSC Sorting

Gentle, Efficient, and Reproducible Single Cell Sorting of Human iPSCs

Human induced pluripotent stem cells (iPSCs) hold immense significance and potential in regenerative medicine and disease modeling. These cells, derived from adult somatic cells like the skin or blood cells, can be reprogrammed to revert to a pluripotent state, which enables them to differentiate into any cell type in the human body (Mahajani et al., 2019)

Application Notes - Neuronal Patterning of Monoclonal iPSCs

Neuronal Patterning of NX One Sorted Pluripotent Monoclonal iPSCs

Human Induced Pluripotent Stem Cells (hiPSCs) are immensely valuable in research and drug discovery. These cells offer a unique platform to study disease mechanisms, model patient-specific conditions, screen potential drug candidates, and test the efficiency of gene editing strategies like CRISPR. Obtaining monoclonal iPSC lines thus becomes crucial

Yeast, nuclei, algae, organoids & more

Accurately sort and characterize a range of particle types as small as yeast and as large as nascent organoids and cell clusters – the NX One’s disposable, contamination-free cartridge supports 2 – 60 µm particles with ease.

Application Notes - Yeast Display Library Screen

Yeast display is an effective method for screening protein binding affinity.

Generating yeast clones through conventional methods such as colony picking and serial dilutions are low throughout, highly labor intensive, and nonspecific

Application Notes - Nodexus Plug and Play Sorting for Large Particles

Plug-and-Play Sorting for Large Particles: 60μm and Beyond

Size-based particle analysis and sorting are crucial in biological applications as the function, origin, or type of biological particles can be traced using cell size as a metric. For traditional fluorescence-activated cell sorting systems, processing large (>30 μm diameter) particles or heterogeneous

Sample Library

CACO-2
T-Cells
Chinese Hamster Ovary (CHO)
HCC1569
HEK293
HeLa
iHEP & other Hepatocytes
iPSC (wild type and Parkinson’s-derived)
Jurkat
K562
L929
MCF7
MDA-MB-231
Primary Human Skeletal Muscle Cells
HT1080
U87
PBMCs
Nascent Organoids
Gibco Viral Production Cells (VPCs)
Keratinocytes
PaCa
U-2 OS
Yeast
Nannochloropsis Algae
Bacteria (E. coli)
Protoplasts
Hydrogels (60-75 micron)
Oil-in-water emulsion droplets (75+ micron)
Mitochondria
Partillion Nanovials

Are you ready to discover more with the NX One?