2 Laser, 4 Channel Configuration (Example)

Laser Wavelength  2 Laser: 405, 488 nm Target Fluorophore(s)
Detector 1
415-455
DAPI, Hoechst, CytoTell, Pacific Blue, Brilliant Violet 421
Detector 2
500-550
GFP, FITC, AF488
Detector 3
550-600
PE, Propidium Iodide
Detector 4
600+
PE-Tandems, Brilliant Violet 605, 645, 711; NovaFluor Violet 690

3 Laser, 4 Channel Configuration (Example)

Laser Wavelength  3 Laser: 405, 488, 638 nm Target Fluorophore(s)
Detector 1
415-455
DAPI, Hoechst, CytoTell, Pacific Blue, Brilliant Violet 421
Detector 2
500-550
GFP, FITC, AF488
Detector 3
550-600
PE, Propidium Iodide
Detector 4
675+
AF647, Cy5, APC, PerCP-tandems, 7-AAD

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